| Autor: |
Pauline Delhez, Elise Meurette, Emilie Knapp, Léonard Theron, Georges Daube, Anne-Sophie Rao |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
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| Zdroj: |
Animals, Vol 11, Iss 6, p 1641 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2076-2615 |
| DOI: |
10.3390/ani11061641 |
| Popis: |
Calves are born agammaglobulinemic and they rely on transfer of passive immunity (TPI) through ingestion of colostrum from the dam. Ensuring the effectiveness of TPI through blood serum immunoglobulins (IgG) quantification is of critical importance for the prevention of calf diseases. The main objective of this study was to assess the performance of a novel on-farm immunochromatographic quick assay (SmartStripsTM, Bio-X Diagnostics, Rochefort, Belgium) compared to the ELISA reference method to directly measure serum IgG concentration and assess TPI status in beef and dairy calves. Additional comparison was made with the commonly used Brix refractometer. Jugular blood samples were collected from beef (n = 71) and dairy (n = 26) calves in Belgium within 7 days post-birth. Quantitative (Pearson correlation coefficients, Bland-Altman plots) and qualitative (diagnostic test characteristics, weighted kappa for classification into 4 categories of TPI) analyses were performed to evaluate the performances of the quick test and the refractometer compared to ELISA. The quick test showed a correlation of 0.83 and a classification agreement (weighted kappa) of 0.79 with the reference method (average values for two types of blood anticoagulants). Performances were better for low IgG concentrations and the assessment of poor TPI status and they outperformed those of the Brix refractometer. Results suggested that the immunochromatographic quick test can be considered as a suitable on-farm method for direct serum IgG measurement and the assessment of TPI status in calves, contributing to timely interventions in the management of calves with inadequate TPI. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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