Combination of Ursodeoxycholic Acid and Glutathione Improves Intestinal Morphology in Cholestasis by Downregulating TNF-α Expression
| Autor: | Jonathan Alvin Nugraha Halim, Endang Sri Lestari, Sigit Adi Prasetyo, Muflihatul Muniroh, Agung Aji Prasetyo |
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| Jazyk: | angličtina |
| Rok vydání: | 2022 |
| Předmět: | |
| Zdroj: | Indonesian Biomedical Journal, Vol 14, Iss 4, Pp 429-35 (2022) |
| Druh dokumentu: | article |
| ISSN: | 2085-3297 2355-9179 |
| DOI: | 10.18585/inabj.v14i4.2044 |
| Popis: | BACKGROUND: Cholestasis caused by obstruction of the common bile duct and may developed gut-derived sepsis due to reactive oxygen species (ROS) accumulation. Ursodeoxycholic acid (UDCA) and glutathione are widely known for their antioxidant properties, that might be beneficial against ROS. However, the effects of UDCA-glutathione combination against ROS have not been well elucidated in previous studies. Thus, this study was conducted to evaluate tumor necrosis factor (TNF)-α level and height of terminal ileal mucosal villus after UDCA-glutathione administration in cholestasis rat model. METHODS: Twenty-eight male Sprague Dawley rats were randomly grouped into four treatment groups, each group consisted of seven rats that had previously undergone bile duct ligation. Three groups received treatment of UDCA-glutathione combination on stratified dose, while the other one only received UDCA. Each treatment was given for 21 days. Ileal samples were collected from the rats and stained with mouse anti TNF-a antibody and hematoxylin-eosin (HE). Immunohistochemistry and histopathological examination were done using microscope and then calculated with ImageJ. RESULTS: The combination of UDCA and glutathione treatment decreased the TNF-α expression (p |
| Databáze: | Directory of Open Access Journals |
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