Confocal scanner laser evaluation of bactericidal effect of different antibiotic mixtures used for dental pulp regeneration: a preliminary study
| Autor: | Genta Elisa, Alovisi Mario, Cuffini Anna Maria, Mandras Narcisa, Luganini Anna, Roana Janira, Pasqualini Daminano, Scotti Nicola, Berutti Elio |
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| Jazyk: | angličtina |
| Rok vydání: | 2016 |
| Předmět: | |
| Zdroj: | Giornale Italiano di Endodonzia, Vol 30, Iss 1, Pp 41-45 (2016) |
| Druh dokumentu: | article |
| ISSN: | 1121-4171 93423691 |
| DOI: | 10.1016/j.gien.2016.04.008 |
| Popis: | Aim: Evaluate the antibacterial efficacy and depth of action into dentinal tubules of different antibiotic mixtures used for dental pulp regeneration (1-9). Methodology: Cylindrical specimens of radicular dentine (n = 72), sterilized with ethylene oxide, have been infected with Enterococcus Faecalis (3 × 107 CFU/mL) for 3 weeks (10). Specimens were randomly assigned to 3 experimental groups (n = 20) plus positive (n = 6) and negative (n = 6) controls. • TRIMIX group (ciprofloxacin, metronidazole and minocycline) • BIMIX group (ciprofloxacin and metronidazole) • TRICLARITRO group (ciprofloxacin, metronidazole and clarithromycin) Each experimental group was divided in two subgroups exposed to antibiotic paste formulations added to macrogol (MG) or ialuronic acid (HA). After 3 weeks o exposition to antibiotic formulations, all specimens were vertically fractured and analyzed with confocal laser scanning microscopy (CLSM) and viability staining (Live/Dead BacLight Viability Stain) to quantitatively evaluate the ratio of dead/live bacteria into dentine tubules (11-12). Volume ratio of red fluorescence (Dead bacteria) was calculated in three-dimensional reconstructions. Differences among groups were analyzed with Kruskall-Wallis and post-hoc Dunn's test (P 0,05). All antibiotic mixtures conveyed by HA showed a better efficacy compared with MG (P 0,05). Conclusions: TRICLARITRO antibiotic mixture showed an effective antibacterial action deep into dentinal tubules, without the risk of tooth crown staining (10). |
| Databáze: | Directory of Open Access Journals |
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