| Autor: |
Elena De Domenico, Lorenzo Bonaguro, Jonas Schulte-Schrepping, Matthias Becker, Kristian Händler, Joachim L. Schultze |
| Jazyk: |
angličtina |
| Rok vydání: |
2020 |
| Předmět: |
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| Zdroj: |
STAR Protocols, Vol 1, Iss 3, Pp 100233- (2020) |
| Druh dokumentu: |
article |
| ISSN: |
2666-1667 |
| DOI: |
10.1016/j.xpro.2020.100233 |
| Popis: |
Summary: In December 2019, a new coronavirus, SARS-CoV-2, which causes the respiratory illness that led to the COVID-19 pandemic, was reported. In the face of such a new pathogen, special precautions must be taken to examine potentially infectious materials due to the lack of knowledge on disease transmissibility, infectivity, and molecular pathogenicity. Here, we present a complete and safe workflow for performing scRNA-seq experiments on blood samples of infected patients from cell isolation to data analysis using the micro-well based BD Rhapsody platform.For complete information on the use and execution of this protocol, please refer to Schulte-Schrepping et al. (2020). |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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