| Autor: |
X.X. Xie, Y.F. Ma, Q.S. Wang, Z.L. Chen, R.R. Liao, Y.C. Pan |
| Jazyk: |
angličtina |
| Rok vydání: |
2015 |
| Předmět: |
|
| Zdroj: |
Brazilian Journal of Medical and Biological Research, Vol 48, Iss 7, Pp 616-621 (2015) |
| Druh dokumentu: |
article |
| ISSN: |
1414-431X |
| DOI: |
10.1590/1414-431X20153848 |
| Popis: |
As an essential trace element, copper can be toxic in mammalian cells when present in excess. Metallothioneins (MTs) are small, cysteine-rich proteins that avidly bind copper and thus play an important role in detoxification. Yeast CUP1 is a member of the MT gene family. The aim of this study was to determine whether yeast CUP1 could bind copper effectively and protect cells against copper stress. In this study, CUP1 expression was determined by quantitative real-time PCR, and copper content was detected by inductively coupled plasma mass spectrometry. Production of intracellular reactive oxygen species (ROS) was evaluated using the 2',7'-dichlorofluorescein-diacetate (DCFH-DA) assay. Cellular viability was detected using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay, and the cell cycle distribution of CUP1 was analyzed by fluorescence-activated cell sorting. The data indicated that overexpression of yeast CUP1 in HeLa cells played a protective role against copper-induced stress, leading to increased cellular viability (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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