| Autor: |
Frank Yu, MSc, Roberto Ribeiro, MD, PhD, Roizar Rosales, MD, Ludger Hauck, PhD, Daniela Grothe, Juglans Alvarez, MD, Mitchell Adamson, MSc, Vivek Rao, MD, PhD, Mitesh Badiwala, MD, PhD, Filio Billia, MD, PhD |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
JHLT Open, Vol 4, Iss , Pp 100074- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2950-1334 |
| DOI: |
10.1016/j.jhlto.2024.100074 |
| Popis: |
Background: Ex situ heart perfusion (ESHP) has been used to optimize donor organs before heart transplantation. However, cardiac function often deteriorates with the development of myocardial edema. The use of dialysis during ESHP could assist in cardiac preservation. Methods: Male Yorkshire pig hearts were subjected to ESHP for 8 hours with or without dialysis. Hearts were supported during nonworking mode and working mode, and pressure-volume loops and coronary vasomotor function were evaluated. Finally, tissue biopsies were assessed for mitochondrial function, oxidative stress, and inflammation. Results: Adding dialysis to ESHP significantly enhanced cardiac function, with improved preload recruitable stroke work at 4 hours (64.09 ± 20.13 vs 35.08 ± 13.52, p = 0.010) and 8 hours (64.31 ± 9.08 vs 23.30 ± 19.25, p = 0.0002), maximal elastance at 8 hours (24.67 ± 10.75 vs 10.62 ± 8.471, p = 0.0477), and end diastolic pressure volume relationship at 8 hours (644.7 ± 566.68 vs 86.63 ± 72.05, p = 0.0187). Coronary vasomotor function improved in the dialysis group in endothelium dependent (LogIC50 −7.39 ± 0.25 vs −2.22 ± 0.76, p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
