| Autor: |
Patricia Brassolatti, Cynthia Aparecida de Castro, Hugo Leonardo dos Santos, Isabelle Taira Simões, Luciana Almeida-Lopes, Juliana Virginio da Silva, Fernanda Oliveira Duarte, Genoveva Lourdes Flores Luna, Wladimir Rafael Beck, Paulo Sergio Bossini, Fernanda de Freitas Anibal |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Acta Cirúrgica Brasileira, Vol 38 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1678-2674 |
| DOI: |
10.1590/acb383823 |
| Popis: |
ABSTRACT Purpose: To evaluate inflammatory response in critical bone injuries after implantation of the biomaterial composed of hydroxyapatite (HA)/poly (lactic-coglycolic acid) (PLGA)/BLEED. Methods: Forty-eight male Wistar rats (280 ± 20 grams) were divided into two groups: control group (CG), in which the animals do not receive any type of treatment; and biomaterial group (BG), in which the animals received the HA/PLGA/BLEED scaffold. Critical bone injury was induced in the medial region of the skull calotte with the aid of a trephine drill 8 mm in diameter. The biomaterial was implanted in the form of 1.5-mm thick scaffolds. Serum and calotte were collected at one, three and seven days. Results: Biomaterial had a significant effect on the morphological structure of the bone, accelerating osteoblast activation within three days, without causing exacerbated systemic inflammation. In addition, quantitative real-time polymerase chain reaction (qRT-PCR) analysis showed that BG induced upregulation of osteogenic genes such as runt-related transcription factor 2, and stimulated genes of inflammatory pathways such as tumor necrosis factor-α, on the first day without overexpressing genes related to bone matrix degradation, such as tissue inhibitor of metalloproteinases-1 and matrix metalloproteinase-9. Conclusions: The HA/PLGA/BLEED® association can be used as a bone graft to aid bone repair, as it is capable of modulating expression of important genes at this stage of the repair process. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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