| Autor: |
A.R. Lavanya, S. Muthukrishnan, M. MuthuKumar, J.H. Franklin Benjamin, T. Senthil Kumar, V. Kumaresan, M.V. Rao |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Journal of Genetic Engineering and Biotechnology, Vol 12, Iss 2, Pp 95-101 (2014) |
| Druh dokumentu: |
article |
| ISSN: |
1687-157X |
| DOI: |
10.1016/j.jgeb.2014.09.002 |
| Popis: |
Hildegardia species are an important resource for fiber industry. This investigation was conducted to develop a plant regeneration protocol for Hildegardia populifolia (Roxb.) Schott & Endl. via indirect organogenesis Callus was obtained from leaf, internode and petiole explants, among these explants internode explant gave best result on MS medium supplemented with different concentrations of 2,4-Dichlorophenoxy acetic acid (2,4-D). The highest percentage (100%) of regeneration was obtained with benzyladenine (BA) (2.0 mg/l) + indole-3-acetic acid (IAA) (0.1 mg/l) + glutamine (25 mg/l) + thidiazuron (TDZ) (0.5 mg/l) from internode explants. Shootlets were highly rooted on MS medium supplemented with 3.0 mg/l indole-3-butyric acid (IBA). In vitro rooted seedlings were successfully acclimatized. This in vitro regeneration system will facilitate further development of reliable procedures for this genus. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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