Exclusive labeling of direct and indirect pathway neurons in the mouse neostriatum by an adeno-associated virus vector with Cre/lox system

Autor: Shinichiro Okamoto, Kenta Yamauchi, Jaerin Sohn, Megumu Takahashi, Yoko Ishida, Takahiro Furuta, Masato Koike, Fumino Fujiyama, Hiroyuki Hioki
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: STAR Protocols, Vol 2, Iss 1, Pp 100230- (2021)
Druh dokumentu: article
ISSN: 2666-1667
DOI: 10.1016/j.xpro.2020.100230
Popis: Summary: We developed an adeno-associated virus (AAV) vector-based technique to label mouse neostriatal neurons comprising direct and indirect pathways with different fluorescent proteins and analyze their axonal projections. The AAV vector expresses GFP or RFP in the presence or absence of Cre recombinase and should be useful for labeling two cell populations exclusively dependent on its expression. Here, we describe the AAV vector design, stereotaxic injection of the AAV vector, and a highly sensitive immunoperoxidase method for axon visualization.For complete details on the use and execution of this protocol, please refer to Okamoto et al. (2020).
Databáze: Directory of Open Access Journals