Antimicrobial Peptide Mechanisms Studied by Whole-Cell Deuterium NMR

Autor:	Sarika Kumari, Valerie Booth
Jazyk:	angličtina
Rok vydání:	2022
Předmět:	whole cell NMR antimicrobial peptides (AMPs) host defense peptides HDPs MSI-78 pexiganan Biology (General) QH301-705.5 Chemistry QD1-999
Zdroj:	International Journal of Molecular Sciences, Vol 23, Iss 5, p 2740 (2022)
Druh dokumentu:	article
ISSN:	23052740 1422-0067 1661-6596
DOI:	10.3390/ijms23052740
Popis:	Much of the work probing antimicrobial peptide (AMP) mechanisms has focussed on how these molecules permeabilize lipid bilayers. However, AMPs must also traverse a variety of non-lipid cell envelope components before they reach the lipid bilayer. Additionally, there is a growing list of AMPs with non-lipid targets inside the cell. It is thus useful to extend the biophysical methods that have been traditionally applied to study AMP mechanisms in liposomes to the full bacteria, where the lipids are present along with the full complexity of the rest of the bacterium. This review focusses on what can be learned about AMP mechanisms from solid-state NMR of AMP-treated intact bacteria. It also touches on flow cytometry as a complementary method for measuring permeabilization of bacterial lipid membranes in whole bacteria.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/66885cf75dc642428ad636728a94aae4 Zobrazit plný text záznamu View record in DOAJ Plný text ve formátu PDF Plný text ve formátu HTML
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