CRISPR-Cas9 cytidine and adenosine base editing of splice-sites mediates highly-efficient disruption of proteins in primary and immortalized cells

Autor:	Mitchell G. Kluesner, Walker S. Lahr, Cara-lin Lonetree, Branden A. Smeester, Xiaohong Qiu, Nicholas J. Slipek, Patricia N. Claudio Vázquez, Samuel P. Pitzen, Emily J. Pomeroy, Madison J. Vignes, Samantha C. Lee, Samuel P. Bingea, Aneesha A. Andrew, Beau R. Webber, Branden S. Moriarity
Jazyk:	angličtina
Rok vydání:	2021
Předmět:	Science
Zdroj:	Nature Communications, Vol 12, Iss 1, Pp 1-12 (2021)
Druh dokumentu:	article
ISSN:	2041-1723
DOI:	10.1038/s41467-021-22009-2
Popis:	Base editors can inactivate splice sites or introduce stop codons into a gene sequence. Here the authors present SpliceR to design, rank, and test sgRNAs for efficient gene disruption in T cells.
Databáze:	Directory of Open Access Journals
Externí odkaz:	https://doaj.org/article/66661828f7814bdf8c8c049d29a4b13b Zobrazit plný text záznamu Full text from SpringerLink View record in DOAJ