Possibilities of investigating intratumoral lymphocyte subpopulations by flow cytometry of tumor core biopsy specimens in patients with breast cancer
| Autor: | F. A. Shamilov, Ya. V. Vishnevskaya, V. Yu. Selchuk, E. M. Pogodina, D. I. Zernov, N. V. Chkhikvadze, V. V. Timoshenko, D. A. Ryabchikov, N. N. Tupitsyn |
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| Jazyk: | ruština |
| Rok vydání: | 2014 |
| Předmět: | |
| Zdroj: | Опухоли женской репродуктивной системы, Vol 0, Iss 3-4, Pp 29-33 (2014) |
| Druh dokumentu: | article |
| ISSN: | 1994-4098 1999-8627 |
| DOI: | 10.17650/1994-4098-2012-0-3-4-29-33 |
| Popis: | Study of tumor-infiltrating lymphocyte subpopulations was performed in 19 patients with breast cancer (core biopsy material). Two probes were used in multiparameter flow cytometry: CD4/CD25/CD3/CD45 and CD8/CD56/CD3/CD45. Mean percentage of different cell types within lymphocytes (CD45++ SSCLow) was as follows: CD3 – 88.2%, CD4 – 45.1%, CD8 – 40.4%, NK (CD3–CD56+) – 1.6%, CD4+CD25+ – 4.1 %. Ratio CD8/CD4 was less than 1 in 53 % of cases. In cases of prominent and moderate CD45 infiltration percentage of CD8+ lymphocytes was significantly higher than in cases with low infiltration, p = 0.007. |
| Databáze: | Directory of Open Access Journals |
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