| Autor: |
Williamson Robin CN, Habib Nagy A, Virlos Ioannis T, Serracino-Inglott Ferdinand, Mathie Robert T |
| Jazyk: |
angličtina |
| Rok vydání: |
2002 |
| Předmět: |
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| Zdroj: |
BMC Gastroenterology, Vol 2, Iss 1, p 22 (2002) |
| Druh dokumentu: |
article |
| ISSN: |
1471-230X |
| DOI: |
10.1186/1471-230X-2-22 |
| Popis: |
Abstract Background Previous work has suggested that in the liver, adenosine preconditioning is mediated by nitric oxide. Whether the endothelial isoform of nitric oxide synthase plays a part in this mechanism has however not yet been investigated. Methods Wistar rats were used (6 in each group) – Groups: (1) sham, (2) ischemia-reperfusion, (3) adenosine + ischemia-reperfusion, (4) endothelial isoform inhibitor + adenosine + ischemia-reperfusion. Results Using immunohistochemistry, this study has revealed a decrease in the expression of endothelial nitric oxide synthase following hepatic ischemia-reperfusion. This was prevented by adenosine pre-treatment. When an inhibitor of endothelial nitric oxide synthase was administered prior to adenosine pre-treatment, pre-conditioning did not occur despite normal expression of endothelial nitric oxide synthase. Conclusions These findings suggest that adenosine attenuates hepatic injury by preventing the downregulation of endothelial nitric oxide synthase that occurs during ischemia-reperfusion. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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