Comparison of HCMV DNA load and antigenemia results in hematopoietic transplant recipients based on GVHD grade
| Autor: | Pajand O, Ziyaeyan M, Mousavi A, Hojabri Z, Kazemi B, Bahador A, Hamidian M, Hashemi F B |
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| Jazyk: | perština |
| Rok vydání: | 2007 |
| Předmět: | |
| Zdroj: | Tehran University Medical Journal, Vol 64, Iss 11, Pp 18-24 (2007) |
| Druh dokumentu: | article |
| ISSN: | 1683-1764 1735-7322 |
| Popis: | Background: Human Cytomegalovirus (HCMV) infections are a significant challenge in patients with Hematopoietic Cell Transplant (HCT). Acute Graft vs. Host (GVHD) is recognized as a predisposing factor for increased incidence of HCMV reactivation. Availability of rapid and accurate tests for HCMV detection in HCT recipients is of foremost importance in developing countries, such as Iran. Methods: A total of 201 peripheral blood leukocyte (PBL) and plasma specimens from 26 allogeneic HCT recipients were examined for HCMV DNA by polymerase chain reaction (PCR) assay. Densitometric analysis of 257bp PCR products from clinical samples and 101-106 "cloned plasmid" per µg DNA containing a HCMV specific fragment were analyzed using LabWorks software (v3.0.02). Optical density of amplicons was plotted, and calculated HCMV viral loads were compared with the patients' antigenemia results. Results: HCMV viral loads ranged between |
| Databáze: | Directory of Open Access Journals |
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