Preculture sugarcane tissue in sucrose-supplemented culture medium to induce desiccation tolerance
| Autor: | Márcio Antônio Rocha Oliveira, Luiz Alexandre Peternelli, Marília Contin Ventrella, Marcone Vieira Sabino, Cristiane Gamarano Melo, Sérgio Yoshimitsu Motoike, Márcio Henrique Pereira Barbosa |
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| Jazyk: | angličtina |
| Rok vydání: | 2011 |
| Předmět: | |
| Zdroj: | Crop Breeding and Applied Biotechnology, Vol 11, Iss 4, Pp 320-329 (2011) |
| Druh dokumentu: | article |
| ISSN: | 1518-7853 1984-7033 |
| Popis: | Cryopreservation is a promising technique for long term conservation of sugarcane’s genetic resources whichcould be used in breeding programs. Preculture is a key step for success this technique. The purpose of this study was toevaluate the effect of sucrose concentrations and preculture period on dessication tolerance of sugarcane tissue dehydrated to30, 20 and 10 % moisture. Sugarcane shoot tips were encapsulated in sodium alginate and precultured in a liquid culturemedium with 0.3, 0.5, and 0.75 M sucrose. Preculturing in 0.3 M sucrose was ideal to induce desiccation tolerance in tissue.This sucrose concentration reduced the percentage of electrolytic leakage. Shoot tips were sensitive to a sucrose concentrationof 0.75 M, resulting in low survival rates after desiccation. Preculturing in 0.5 M sucrose resulted in a higher survival rateafter drying for 5.7 and 7.45 h. The percentage of electrolytic leakage was high when shoot tips were desiccated afterpreculturing in 0.5 and 0.75 M sucrose. |
| Databáze: | Directory of Open Access Journals |
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