MILT DILUTION EFFECTIVENESS ON PIKEPERCH (SANDER LUCIOPERCA) SPERM DNA INACTIVATION
| Autor: | B. KORBULY, A. GROZEA, ADA CEAN, I. BĂNĂŢEAN-DUNEA, N. PĂCALĂ, A. VĂLEAN |
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| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | Scientific Papers Animal Science and Biotechnologies, Vol 42, Iss 2, Pp 65-65 (2023) |
| Druh dokumentu: | article |
| ISSN: | 1841-9364 2344-4576 |
| Popis: | Percid fishes, including pikeperch (Sander lucioperca) have recently become the subject of intense research. In order to obtain gynogenetic all female pikeperch populations, normal pikeperch eggs are fertilized with inactivated sperm. Because pikeperch semen has a high viscosity, milt has to be diluted in an immobilizing solution before DNA inactivation. The aim of this study was to assess milt diluting solutions effectiveness in order to inactivate sperm DNA with UV irradiation, to produce meiotic gynogenetic pikeperch (Sander lucioperca). We assessed sperm motility after dilution in 5 different immobilizing solutions. Best results were obtained using Ringer’s solution as pikeperch sperm diluent. |
| Databáze: | Directory of Open Access Journals |
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