Analysis of the distribution of ficolin gene polymorphisms (FCN2) in children with bronchial asthmas of various severity
| Autor: | M. V. Smolnikova, K. V. Afonicheva, I. V. Marchenko, S. Yu. Tereshchenko |
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| Jazyk: | ruština |
| Rok vydání: | 2024 |
| Předmět: | |
| Zdroj: | Медицинская иммунология, Vol 26, Iss 4, Pp 727-732 (2024) |
| Druh dokumentu: | article |
| ISSN: | 1563-0625 2313-741X |
| DOI: | 10.15789/1563-0625-AOT-16854 |
| Popis: | Asthma is one of the most common chronic diseases in all age groups. Asthma has heterogeneous phenotypes with different etiologies. Many parameters are used to classify asthma, for example, the severity and level of flow control. The asthma phenotype is dependent on the state of the immune system, and innate immunity plays an important role in the susceptibility and pathophysiology of asthma. The complement system (CS) consists of a complex of protective proteolytic enzymes (including lectins). Ficolin-2 (L-ficolin) is one of the main opsonizing molecules of respiratory secretions and a protein of the lectin pathway of CS activation. Polymorphisms in the L-ficolin gene affect the level of expression which may be associated with a higher susceptibility to infections and viruses, as well as a predisposition to asthma.Aim: To study the distribution of polymorphisms rs17549193 and rs7851696 of the L-ficolin (FCN2) gene in children with asthma of varying severity.Russian children from the Children’s Allergy Center (Krasnoyarsk, Russia), aged from 8 to 18 years, were studied. Children with asthma were divided into groups depending on the severity of the disease in accordance with GINA-2023: mild (n = 146) and severe (n = 254). The comparison group included children of comparable age and gender without asthma, allergies or infections. DNA extraction from blood was performed using the sorbent method. Genotyping of polymorphisms rs17549193 and rs7851696 FCN2 was performed by real-time polymerase chain reaction.The results obtained provide distribution of the polymorphic variants FCN2 gene in the population of healthy Russian children and in children with a socially and economically important disease, namely asthma. The distribution of rs17549193 and rs7851696 FCN2 corresponds to the global Caucasoid populations. There were no statistically significant differences between asthma patients with varying degrees of severity of the disease and healthy ones in the studied sample.The results indicate an expansion of the sample and range of studied polymorphic genes of proteins of the lectin pathway of CS activation due to their importance for the prevention of severe forms of diseases, as well as their significance in the functioning of the immune system. |
| Databáze: | Directory of Open Access Journals |
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