Optimization and modification of the method for detection of rhamnolipids
| Autor: | Takeshi Tabuchi, Diandra Martínez, Manuel Hospinal, Fernando Merino, Susana Gutiérrez |
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| Jazyk: | English<br />Spanish; Castilian |
| Rok vydání: | 2015 |
| Předmět: | |
| Zdroj: | Revista Peruana de Biología, Vol 22, Iss 2, Pp 247-258 (2015) |
| Druh dokumentu: | article |
| ISSN: | 1561-0837 1727-9933 |
| DOI: | 10.15381/rpb.v22i2.11359 |
| Popis: | Use of biosurfactants in bioremediation, facilitates and accelerates microbial degradation of hydrocarbons. CTAB/MB agar method created by Siegmund & Wagner for screening of rhamnolipids (RL) producing strains, has been widely used but has not improved significantly for more than 20 years. To optimize the technique as a quantitative method, CTAB/MB agar plates were made and different variables were tested, like incubation time, cooling, CTAB concentration, methylene blue presence, wells diameter and inocula volume. Furthermore, a new method for RL detection within halos was developed: precipitation of RL with HCl, allows the formation a new halos pattern, easier to observe and to measure. This research reaffirm that this method is not totally suitable for a fine quantitative analysis, because of the difficulty to accurately correlate RL concentration and the area of the halos. RL diffusion does not seem to have a simple behavior and there are a lot of factors that affect RL migration rate. |
| Databáze: | Directory of Open Access Journals |
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