Molecular Determination of Toxigenic Potential of Fusarium spp. Isolated from Seeds of Wheat (Triticum aestivum) Genotypes and Evaluation of Levels of Fumonisins in the Grains at Harvest in Three Major Wheat Producing Counties in Kenya

Autor: Phanice Kheseli Otieno, Susan S. Imbahale, Vitalis Wafula Wekesa, Miriam Otipa, Sheila Okoth
Jazyk: angličtina
Rok vydání: 2022
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Zdroj: International Journal of Agronomy, Vol 2022 (2022)
Druh dokumentu: article
ISSN: 1687-8167
DOI: 10.1155/2022/1428312
Popis: Infestation of cereals such as wheat by pathogenic Fusarium species (Fusarium spp.) is associated with over 25% weight loss of the grain and accumulation of hazardous mycotoxins. Potential management strategies against the pathogens include the development of more resistant wheat genotypes and the development of rapid and accurate Fusarium spp. identification methods, among other control measures. This study evaluated the toxigenic potential in populations of Fusarium spp. isolated from seeds of locally developed wheat genotypes and levels of fumonisins in the grains at harvest in three counties in Kenya. The sampling of the wheat grains took place between September 2016 and October 2017. Determination of toxigenic potential was PCR based using Tri13F/Tri13DONR and FUM1F/FUM1R specific primer pairs while detection of fumonisin levels was done using Total Fumonisin Assay 0.25/5.0 ELISA kit. Data were analyzed using ANOVA and the Tukey HSD test. FUM1 gene was detected in 60% of the Fusarium spp. analyzed. The distribution of the gene within the isolates across the three regions was as follows: Narok: 54%, Uasin Gishu: 25%, and Nakuru: 21%. Tri13DON gene was not detected in the assessed potential deoxynivalenol (DON) producers. Fumonisin levels in the wheat samples from the three counties were significantly different (p
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