| Autor: |
Gollapudi Sastry, Matsuura Motohiro, Ghoneum Mamdooh |
| Jazyk: |
angličtina |
| Rok vydání: |
2009 |
| Předmět: |
|
| Zdroj: |
Nutrition Journal, Vol 8, Iss 1, p 18 (2009) |
| Druh dokumentu: |
article |
| ISSN: |
1475-2891 |
| DOI: |
10.1186/1475-2891-8-18 |
| Popis: |
Abstract Background Several studies have examined the correlation between iron oxidation and H2O2 degradation. The present study was carried out to examine the protective effects of MRN-100 against stress-induced apoptosis in murine splenic cells in vitro. MRN-100, or HydroFerrate fluid, is an iron-based beverage composed of bivalent and trivalent ferrates. Methods Splenic lymphocytes from mice were cultured in the presence or absence of MRN-100 for 2 hrs and were subsequently exposed to hydrogen peroxide (H2O2) at a concentration of 25 μM for 14 hrs. Percent cell death was examined by flow cytometry and trypan blue exclusion. The effect of MRN-100 on Bcl-2 and Bax protein levels was determined by Western blot. Results Results show, as expected, that culture of splenic cells with H2O2 alone results in a significant increase in cell death (apoptosis) as compared to control (CM) cells. In contrast, pre-treatment of cells with MRN-100 followed by H2O2 treatment results in significantly reduced levels of apoptosis. In addition, MRN-100 partially prevents H2O2-induced down-regulation of the anti-apoptotic molecule Bcl-2 and upregulation of the pro-apoptotic molecule Bax. Conclusion Our findings suggest that MRN-100 may offer a protective effect against oxidative stress-induced apoptosis in lymphocytes. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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