BIOFILM FORMATION AND MOTILITY OF BACTERIA PSEUDOMONAS AERUGINOSA WITH DIFFERENT C-DI- GMPLEVEL

Autor: М. Б. Галкін, А. С. Семенець, М. О. Фіногенова, Б. М. Галкін, Т. О. Філіпова
Jazyk: English<br />Russian<br />Ukrainian
Rok vydání: 2017
Předmět:
Zdroj: Mìkrobìologìâ ì Bìotehnologìâ, Vol 0, Iss 2(38), Pp 40-50 (2017)
Druh dokumentu: article
ISSN: 2076-0558
2307-4663
DOI: 10.18524/2307-4663.2017.2(38).105020
Popis: Aim: Biofilm formation abilities determination in Pseudomonasaeruginosa strains with different cyclic dimeric guanosine monophosphate (c-di-GMP) level and swimming, swarming, and twitching motilities. Materials and methods. Wilde type strain P. aeruginosa PA01 and strains with low (PA01 pJN2133) and high (PA01 ΔwspF1) level of c-di-GMP were used as test-organisms. Bacteria were cultivated in 24-walls Nuclon plates in LB medium at 37 °С for 24 hours for biofilm formation. Determination of planktonic cells amount was carried out spectrophotometrically, biofilm formation – by CV-test. Cells motility was tested on Petri dishes with different agar content: swimming 0.3%, swarming 0.6%, and twitching 1.5%. Results. It was shown that P. aeruginosa PA01 pJN2133 form biofilm with impaired structure and in 3.7 and 5 times less intensive than P. aeruginosa PA01 and PA01 ΔwspF1. P. aeruginosa PA01 pJN2133 planktonic cells amount was higher than in wild type strain and PA01 ΔwspF1. According to the morphology of a biofilm of strains P. aeruginosa PA01 and PA01 ΔwspF1 were similar: they contained 3D structures, which in the case of the wild type strain were larger and more clearly separated from each other. The biofilm P. aeruginosa PA01 pJN2133 evenly covered the surface, was very thin and did not contain three-dimensional components. It is shown that cells of P. aeruginosa PA01 pJN2133 strain are the most mobile in all types of surface movement. Strains of P. aeruginosa PA01 and PA01 ΔwspF1 possessed the same activity when moving by swimming and twitching, but differed in their ability to swarm – in P. aeruginosa PA01 ΔwspF1 this process is blocked. P. aeruginosa PA01 pJN2133 swarming motility zones diameter was 62 мм and it were in 1,4 times higher than at P. aeruginosa PA01 – 43 мм. Swarming motility zones morphology was different. Conclusions. The low intracellular content of c-di-GMP interferes with the formation of a full-fledged biofilm and promotes the planktonic mode of P. aeruginosa PA01 pJN2133 and the active movement of cells across different surfaces. An increased level of c-di-GMP provides the formation of a biofilm with a larger mass compared to the control strain and inhibits the swarming process in P. aeruginosa PA01 ΔwspF1.
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