| Autor: |
Nur Alia Sheh Omar, Yap Wing Fen, Jaafar Abdullah, Che Engku Noramalina Che Engku Chik, Mohd Adzir Mahdi |
| Jazyk: |
angličtina |
| Rok vydání: |
2018 |
| Předmět: |
|
| Zdroj: |
Sensing and Bio-Sensing Research, Vol 20, Iss , Pp 16-21 (2018) |
| Druh dokumentu: |
article |
| ISSN: |
2214-1804 |
| DOI: |
10.1016/j.sbsr.2018.06.001 |
| Popis: |
Application of surface plasmon resonance (SPR) optical sensor in the diagnosis of dengue virus (DENV) emerged over recent years. Immobilized monoclonal antibody (IgM) on gold/Fe-MPA-NCC-CTAB/EDC-NHS thin film was prepared using a spin coating technique. DENV E-protein can be detected by measuring the SPR signal when IgM immobilized gold/Fe-MPA-NCC-CTAB/EDC-NHS thin film is exposed to the DENV E-protein solution, by varying the concentration ranging from 0.0001 nM to 10 nM. A linear relationship between the shift of SPR angle and concentration of DENV E-protein up to 0.01 nM, with sensitivity of 39.96° nM−1 has been observed. The surface morphology of sensor chip was also recorded using atomic force microscopy to confirm the presence of bound DENV E-protein into IgM surface. Keywords: Optical sensor, Surface plasmon resonance, Sensing, Dengue virus |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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