Alpha lipoic acid improves heat stress-induced reduction of serum oestradiol and progesterone levels by affecting oxidative and endoplasmic reticulum stress in hens

Autor: Yongjie Xiong, Qirun Yin, Kai Song, Jing Li, Shaojun He
Jazyk: angličtina
Rok vydání: 2021
Předmět:
Zdroj: Czech Journal of Animal Science, Vol 66, Iss 5, Pp 179-187 (2021)
Druh dokumentu: article
ISSN: 1212-1819
1805-9309
DOI: 10.17221/241/2020-CJAS
Popis: Alpha lipoic acid (ALA), a natural lipophilic compound, plays an important role in regulating several metabolic pathways due to its antioxidant properties. This study aims to investigate whether ALA could be used as a feed additive to enhance the antioxidant capacity of the ovary tissue in hens exposed to heat stress (HS). One hundred and sixty 128-days-old female chickens were randomly assigned into four groups: the control group (Con), ALA treatment group (ALA), ALA and HS treatment group (ALA + HS), and HS treatment group (HS). The ALA and ALA + HS groups were fed a basal diet with 0.25% ALA, whereas the Con and HS groups were fed a basal diet only. Serum oestradiol, progesterone levels, biomarkers of antioxidant capacity, and endoplasmic reticulum (ER) stress markers were detected in the ovaries of heat-stressed chickens. HS decreased serum oestradiol and progesterone concentrations compared with the control group, whereas dietary ALA (0.25%) increased oestradiol and progesterone levels in the serum of heat-stressed hens. Malondialdehyde concentration in the ovary was higher in the HS group than that of the ALA + HS group. Compared with the HS group, ALA increased the enzymatic activities of superoxide dismutase, glutathione peroxidase, and catalase in the ovaries of ALA + HS group. Simultaneously, ALA enhanced the total antioxidative capacity of the ovaries of heat-stressed hens. Moreover, ALA also significantly inhibited the increased expression of glucose-regulated protein 78 and CCAAT/enhancer-binding protein homologous protein, which are two markers of ER stress, and heat shock protein 70, a key biomarker of heat stress, in the ovaries of the ALA + HS group as compared to those of the HS group. This work implied that dietary ALA supplementation improved the antioxidant capacity and attenuated the HS-induced reduction of serum oestradiol and progesterone levels and modulated the oxidative and ER stress, which are involved in the protective effect of ALA in hens exposed to hyperthermia.
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