Multivariate Method Based on Raman Spectroscopy for Quantification of Dipyrone in Oral Solutions

Autor: Luciana Lopes Guimarães, Letícia Parada Moreira, Bárbara Faria Lourenço, Walber Toma, Renato Amaro Zângaro, Marcos Tadeu Tavares Pacheco, Landulfo Silveira
Jazyk: angličtina
Rok vydání: 2018
Předmět:
Zdroj: Journal of Spectroscopy, Vol 2018 (2018)
Druh dokumentu: article
ISSN: 2314-4920
2314-4939
DOI: 10.1155/2018/3538171
Popis: This work employed a quantitative model based on Raman spectroscopy and principal component regression (RS/PCR) to quantify the active ingredient dipyrone (metamizole) in commercially available formulations as an analytical methodology for quality control in the pharmaceutical industry. Raman spectra were collected using a dispersive Raman spectrometer (830 nm, 250 mW excitation, and 20 s exposure time) coupled to a Raman probe. Solutions of dipyrone diluted in water in the range of 80 to 120% of the concentration of commercial formulations (500 mg/mL) were used to develop a calibration model based on PCR to obtain the figures of merit for class I validation from the Brazilian Sanitary Surveillance Agency (ANVISA, RE no. 899/2003). This spectral model was then used to predict the concentration of dipyrone in commercial formulations from distinct brands with 500 mg/mL. A prediction error of 6.5 mg/mL (1.3%) was found for this PCR model using the diluted samples. Commercial formulations had predicted concentrations with a difference below 5.0% compared to the label concentration, indicating the applicability of Raman spectroscopy for quality control in the final product.
Databáze: Directory of Open Access Journals
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