Establishment of a primary cell culture of Thrips palmi (Thysanoptera: Thripidae)
| Autor: | Amalendu GHOSH, Heena DHALL, Sumit JANGRA |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2023 |
| Předmět: | |
| Zdroj: | European Journal of Entomology, Vol 120, Iss 1, Pp 297-303 (2023) |
| Druh dokumentu: | article |
| ISSN: | 1210-5759 1802-8829 54143918 |
| DOI: | 10.14411/eje.2023.030 |
| Popis: | Thrips palmi (Thysanoptera: Thripidae) is an important pest of vegetables, ornamental plants and fruit crops. In addition to the direct damage caused by feeding, it transmits several tospoviruses. The absence of an in vitro assay system is a major bottleneck in investigating thrips-tospovirus interactions. The present study reports the establishment of a primary cell culture of T. palmi, which was initiated using embryonic tissue as an explant in modified Kimura's medium. Fibroblast-like cells began to be produced 3 h after tissue implantation and were the dominant cell type. They grew in size and number and covered most of the surface. This primary cell culture survived for 37 days providing sufficient time for analytical molecular studies on the replication of tospovirus and interactions with the vector components. |
| Databáze: | Directory of Open Access Journals |
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