Characterization of rumen microbiota in lactating Holstein cows fed molasses versus corn grain at two levels of rumen-degradable protein

Autor: E. Guduk, M. B. Hall, G. I. Zanton, A. J. Steinberger, P. J. Weimer, G. Suen, K. A. Weigel
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Frontiers in Microbiomes, Vol 2 (2023)
Druh dokumentu: article
ISSN: 2813-4338
DOI: 10.3389/frmbi.2023.1204988
Popis: We evaluated the influence of diets differing in non-fiber carbohydrates and rumen-degradable protein (RDP) levels on changes in the ruminal bacterial populations in lactating Holstein cows. In all, 12 ruminally cannulated cows were assigned to diets with high or low RDP levels. Within each RDP level, molasses was substituted for corn grain at a concentration of 0%, 5.25%, or 10.5% of diet dry matter in a replicated 3 × 3 Latin square design with 28-day periods. Liquid and solid rumen digesta fractions collected at the end of each period underwent 16S rRNA gene sequencing to identify operational taxonomic units and were analyzed for short-chain fatty acids. Protein degradability affected 6 bacterial genera, whereas carbohydrate alteration impacted 13 genera (p
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