| Autor: |
Gridina Maria, Popov Andrey, Shadskiy Artem, Torgunakov Nikita, Kechin Andrey, Khrapov Evgeny, Ryzhkova Oxana, Filipenko Maxim, Fishman Veniamin |
| Jazyk: |
angličtina |
| Rok vydání: |
2023 |
| Předmět: |
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| Zdroj: |
Epigenetics & Chromatin, Vol 16, Iss 1, Pp 1-13 (2023) |
| Druh dokumentu: |
article |
| ISSN: |
1756-8935 |
| DOI: |
10.1186/s13072-023-00524-4 |
| Popis: |
Abstract This study presents a novel approach for mapping global chromatin interactions using S1 nuclease, a sequence-agnostic enzyme. We develop and outline a protocol that leverages S1 nuclease's ability to effectively introduce breaks into both open and closed chromatin regions, allowing for comprehensive profiling of chromatin properties. Our S1 Hi–C method enables the preparation of high-quality Hi–C libraries, marking a significant advancement over previously established DNase I Hi–C protocols. Moreover, S1 nuclease's capability to fragment chromatin to mono-nucleosomes suggests the potential for mapping the three-dimensional organization of the genome at high resolution. This methodology holds promise for an improved understanding of chromatin state-dependent activities and may facilitate the development of new genomic methods. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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