| Autor: |
Rui Wang, Xixi Liu, Li Li, Ming Yang, Jun Yong, Fan Zhai, Lu Wen, Liying Yan, Jie Qiao, Fuchou Tang |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
|
| Zdroj: |
Genomics, Proteomics & Bioinformatics, Vol 20, Iss 2, Pp 223-245 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
1672-0229 |
| DOI: |
10.1016/j.gpb.2022.04.002 |
| Popis: |
Gonadal somatic cells are the main players in gonad development and are important for sex determination and germ cell development. Here, using a time-series single-cell RNA sequencing (scRNA-seq) strategy, we analyzed fetal germ cells (FGCs) and gonadal somatic cells in human embryos and fetuses. Clustering analysis of testes and ovaries revealed several novel cell subsets, including POU5F1+SPARC+ FGCs and KRT19+ somatic cells. Furthermore, our data indicated that the bone morphogenetic protein (BMP) signaling pathway plays cell type-specific and developmental stage-specific roles in testis development and promotes the gonocyte-to-spermatogonium transition (GST) in late-stage testicular mitotic arrest FGCs. Intriguingly, testosterone synthesis function transitioned from fetal Sertoli cells to adult Leydig cells in a stepwise manner. In our study, potential interactions between gonadal somatic cells were systematically explored and we identified cell type-specific developmental defects in both FGCs and gonadal somatic cells in a Turner syndrome embryo (45, XO). Our work provides a blueprint of the complex yet highly ordered development of and the interactions among human FGCs and gonadal somatic cells. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
|
