Label-free cell cycle analysis for high-throughput imaging flow cytometry

Autor: Thomas Blasi, Holger Hennig, Huw D. Summers, Fabian J. Theis, Joana Cerveira, James O. Patterson, Derek Davies, Andrew Filby, Anne E. Carpenter, Paul Rees
Jazyk: angličtina
Rok vydání: 2016
Předmět:
Zdroj: Nature Communications, Vol 7, Iss 1, Pp 1-9 (2016)
Druh dokumentu: article
ISSN: 2041-1723
DOI: 10.1038/ncomms10256
Popis: Imaging flow cytometry enables high-throughput acquisition of fluorescence, brightfield and darkfield images of biological cells. Here, Blasi et al.demonstrate that applying machine learning algorithms on brightfield and darkfield images can detect cellular phenotypes without the need for fluorescent stains, enabling label-free assays.
Databáze: Directory of Open Access Journals