| Autor: |
David O Oladejo, Dorathy O Anzaku, Collins O Mamudu, Temitope Elugbadebo, Titilope M Dokunmu, Ezekiel F Adebiyi, Emeka EJ Iweala |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
|
| Zdroj: |
Scientific African, Vol 23, Iss , Pp e02010- (2024) |
| Druh dokumentu: |
article |
| ISSN: |
2468-2276 |
| DOI: |
10.1016/j.sciaf.2023.e02010 |
| Popis: |
Resistance to antimalarial drugs leads a global recurrence of malaria, posing a significant challenge to malaria control. This study aimed to assess gene expression and resistance profiles in clinical isolates, and validate the inhibitory effect of MCL compound against PbAP2-I and PbBDP1 genes in vivo. Malaria prevalence rate throughout the clinical study period stood at 12.24%. Blood samples were obtained from 182 malaria outpatients and 30 P. berghei-infected mice treated with varying MCL concentrations. DNA and RNA were extracted. PfAP2-I, PfBDP1, PfMDR1, and PfK13 genes were amplified using Real-time qPCR and sequenced for mutation profiling. Relative gene expression of AP2-I and BDP1 genes in P. falciparum and P. berghei-treated mice was carried out by Reverse-transcription PCR. The data were analyzed using ANOVA at P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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