Differential adhesion and fibrinolytic activity of mesenchymal stem cells from human bone marrow, placenta, and Wharton’s jelly cultured in a fibrin hydrogel

Autor: Casandra P Chaires-Rosas, Xóchitl Ambriz, Juan J Montesinos, Beatriz Hernández-Téllez, Gabriela Piñón-Zárate, Miguel Herrera-Enríquez, Érika Hernández-Estévez, Javier R Ambrosio, Andrés Castell-Rodríguez
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Journal of Tissue Engineering, Vol 10 (2019)
Druh dokumentu: article
ISSN: 2041-7314
20417314
DOI: 10.1177/2041731419840622
Popis: Mesenchymal stem cells isolated from different tissues should share associated markers and the capability to differentiate to mesodermal lineages. However, their behavior varies in specific microenvironments. Herein, adhesion and fibrinolytic activity of mesenchymal stem cells from placenta, bone marrow, and Wharton’s jelly were evaluated in fibrin hydrogels prepared with nonpurified blood plasma and compared with two-dimensional cultures. Despite the source, mesenchymal stem cells adhered through focal adhesions positive for vinculin and integrin αV in two dimensions, while focal adhesions could not be detected in fibrin hydrogels. Moreover, some cells could not spread and stay rounded. The proportions of elongated and round phenotypes varied, with placenta mesenchymal stem cells having the lowest percentage of elongated cells (~10%). Mesenchymal stem cells degraded fibrin at distinct rates, and placenta mesenchymal stem cells had the strongest fibrinolytic activity, which was achieved principally through the plasminogen–plasmin axis. These findings might have clinical implications in tissue engineering and wound healing therapy.
Databáze: Directory of Open Access Journals
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