| Autor: |
Clara Fabris, Adriana Abrami, Daiana Bevilacqua, Tiziana Bordon, Franca Cian, Bruno Biasioli |
| Jazyk: |
English<br />Italian |
| Rok vydání: |
2010 |
| Předmět: |
|
| Zdroj: |
Microbiologia Medica, Vol 25, Iss 1 (2010) |
| Druh dokumentu: |
article |
| ISSN: |
2280-6423 |
| DOI: |
10.4081/mm.2010.2484 |
| Popis: |
The IGRAs (Interferon-γ Release Assays) are commercial assays which measure T-cell-mediated response in vitro after specific stimulation, and represent the alternative to TST (Tuberculin Skin Test) in the diagnosis of latent tuberculosis infection (ILTB) and of active tuberculosis (TB). Among these we choose the assay QuantiFERON ®-TB Gold IT (Cellestis Ltd, Carnegie,Victoria, Australia) (QFT) in which interferon-γ (IFN-γ) was measured after lymphocyte stimulation with 3 antigens of the RD1 region of M. tuberculosis: ESAT-6, CFP-10 and TB 7.7. We wanted to assess the sensitivity of the method in cases of pulmonary TB, using cultured isolates of M. tuberculosis as gold-standard. From August 2006 to July 2009 there were 54 suspected cases of pulmonary TB, confirmed by culture.The QFT was requested for 32 patients before any microbiological confirmation and therapy. Of these patients 14 aged 20-50 years, 6 aged 50-70 years and 12 were more than 70-year-old. The QFT was positive in 25 patients (INF-γ> 0.35 IU/mL) and negative in 7 patients (INF-γ |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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