| Autor: |
Marek Dziadosz, Rüdiger Lessig, Heidemarie Bartels |
| Jazyk: |
angličtina |
| Rok vydání: |
2014 |
| Předmět: |
|
| Zdroj: |
Egyptian Journal of Forensic Sciences, Vol 4, Iss 2, Pp 50-53 (2014) |
| Druh dokumentu: |
article |
| ISSN: |
2090-536X |
| DOI: |
10.1016/j.ejfs.2013.12.001 |
| Popis: |
This study describes the possibility of handling Taxus baccata intoxications by the use of an LC–MS/MS method for the direct confirmation of taxine B. A simple and fast preparation procedure of a methanol yew leaves’ extract was applied. This extract made the LC–MS/MS taxine B/isotaxine B optimization possible by the use of its continuous infusion. The analyses were performed with an Agilent 1100 Series HPLC-API 2000™ LC–MS/MS system. The following mass-transitions were monitored in the positive MRM mode: 584.4/194.1 and 584.4/107.2. A Zorbax SB-C18, 4.6 × 50 mm, 5 micron column maintained at 45 ± 1 °C was used for the separation. 20 μL of a prepared sample was injected into the HPLC and eluted with a mobile phase consisting of A: CH3OH and B: 50 mL 0.1 M CH3COONH4 + 50 mL CH3CN + 900 mL H2O + 1 mL CH3COOH (A:B = 7:3, v/v). The flow rate was 0.3 mL/min and a single LC–MS/MS run was completed in 20 min. The biological material was extracted with Extrelut® NT 3 extraction tubes. In fatalities, where a T. baccata intoxication was indicated by the presence of 3,5-dimethoxyphenol in biological material, taxine B/isotaxine B was also identified in the appropriate post-mortem specimens. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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