Immuno-PET imaging for non-invasive assessment of cetuximab accumulation in non-small cell lung cancer

Autor: Aiko Yamaguchi, Arifudin Achmad, Hirofumi Hanaoka, Yusri Dwi Heryanto, Anu Bhattarai, Ratianto, Erdene Khongorzul, Rini Shintawati, A. Adhipatria P. Kartamihardja, Ayaka Kanai, Yumi Sugo, Noriko S. Ishioka, Tetsuya Higuchi, Yoshito Tsushima
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: BMC Cancer, Vol 19, Iss 1, Pp 1-8 (2019)
Druh dokumentu: article
ISSN: 1471-2407
33388415
DOI: 10.1186/s12885-019-6238-4
Popis: Abstract Backgrounds Overexpression of epidermal growth factor receptor (EGFR) has been established as a valid therapeutic target of non-small cell lung cancer (NSCLC). However, the clinical benefit of cetuximab as an EGFR-targeting drug is still controversial, partially due to the lack of effective means to identify suitable patients. This study aimed to investigate the potential of radiolabeled cetuximab as a non-invasive tool to predict cetuximab accumulation in NSCLC tumor xenografts with varying EGFR expression levels. Methods The NSCLC tumors in model mice were subjected to in vivo biodistribution study and positron emission tomography (PET) imaging 48 h after injection of either 111In- or 64Cu-labeled cetuximab. The EGFR expression levels of NSCLC tumors were determined by ex vivo immunoblotting. Results We found that tumors with high EGFR expression had significantly higher [111In]In-DOTA-cetuximab accumulation than tumors with moderate to low EGFR expression (P
Databáze: Directory of Open Access Journals
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