Time to resolution of airway inflammation caused by bronchoalveolar lavage in healthy horses

Autor: Jane S. Woodrow, Klaus Hopster, Megan Palmisano, Flavie Payette, Jeaneen Kulp, Darko Stefanovski, Rose Nolen‐Walston
Jazyk: angličtina
Rok vydání: 2024
Předmět:
Zdroj: Journal of Veterinary Internal Medicine, Vol 38, Iss 5, Pp 2776-2782 (2024)
Druh dokumentu: article
ISSN: 1939-1676
0891-6640
DOI: 10.1111/jvim.17169
Popis: Abstract Background Bronchoalveolar lavage (BAL) is a common procedure for evaluation of the equine lower airways. Time to resolution of post‐BAL inflammation has not been clearly defined. Hypothesis Residual inflammation, evident by changes in immune cell populations and inflammatory cytokines, will resolve by 72 hours after BAL. Animals Six adult, healthy, institution‐owned horses. Methods Randomized, complete cross‐over design. Each horse underwent 3 paired BALs, including a baseline and then 48, 72, and 96 hours later, with a 7‐day washout between paired BALs. Each sample underwent cytological evaluation and cytokine concentrations were determined by a commercially available multiplex bead immunoassay. Statistical analysis was performed by multilevel mixed‐effects Poisson regression analysis. Data are reported as marginal means and 95% confidence interval (CI). Results Neutrophil, eosinophil and mast cell percentages were not significantly different at any time points. Macrophage percentages were higher at 72 hours (45.0 [95% CI, 41.6‐48.4]%) and 96 hours (45.3 [95% CI, 42.9‐47.7]%) vs baseline (37.4 [95% CI, 33.5‐41.4]%; P
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