Two Loci, RiAF3 and RiAF4, Contribute to the Annual-Fruiting Trait in Rubus

Autor: Rubina Jibran, Jessica Spencer, Gina Fernandez, Amparo Monfort, Mourad Mnejja, Helge Dzierzon, Jibran Tahir, Kevin Davies, David Chagné, Toshi M. Foster
Jazyk: angličtina
Rok vydání: 2019
Předmět:
Zdroj: Frontiers in Plant Science, Vol 10 (2019)
Druh dokumentu: article
ISSN: 1664-462X
DOI: 10.3389/fpls.2019.01341
Popis: Most Rubus species have a biennial cycle of flowering and fruiting with an intervening period of winter dormancy, in common with many perennial fruit crops. Annual-fruiting (AF) varieties of raspberry (Rubus idaeus and Rubus occidentalis L.) and blackberry (Rubus subgenus Rubus) are able to flower and fruit in one growing season, without the intervening dormant period normally required in biennial-fruiting (BF) varieties. We used a red raspberry (R. idaeus) population segregating for AF obtained from a cross between NC493 and ‘Chilliwack’ to identify genetic factors controlling AF. Genotyping by sequencing (GBS) was used to generate saturated linkage maps in both parents. Trait mapping in this population indicated that AF is controlled by two newly identified loci (RiAF3 and RiAF4) located on Rubus linkage groups (LGs) 3 and 4. The location of these loci was analyzed using single-nucleotide polymorphism (SNP) markers on independent red raspberry and blackberry populations segregating for the AF trait. This confirmed that AF in Rubus is regulated by loci on LG 3 and 4, in addition to a previously reported locus on LG 7. Comparative RNAseq analysis at the time of floral bud differentiation in an AF and a BF variety revealed candidate genes potentially regulating the trait.
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