Genotyping of methicillin-resistant Staphylococcus aureus isolates obtained in the Northeast region of Brazil
| Autor: | F.C. de Sousa-Junior, M.C. Silva-Carvalho, M.J.B.C. Fernandes, M.F.P. Vieira, F.L.P.C. Pellegrino, A.M.S. Figueiredo, M.C.N. de Melo, E.P. Milan |
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| Jazyk: | angličtina |
| Rok vydání: | 2009 |
| Předmět: | |
| Zdroj: | Brazilian Journal of Medical and Biological Research, Vol 42, Iss 10, Pp 877-881 (2009) |
| Druh dokumentu: | article |
| ISSN: | 0100-879X 1414-431X 01439197 |
| Popis: | Methicillin-resistant Staphylococcus aureus (MRSA) is a major agent of hospital infections worldwide. In Brazil, a multiresistant MRSA lineage (ST239-SCCmecIIIA), the so-called Brazilian epidemic clone (BEC), has predominated in all regions. However, an increase in nosocomial infections caused by non-multiresistant MRSA clones has recently been observed. In the present study, 45 clinical isolates of MRSA obtained from a university hospital located in Natal city, Brazil, were identified by standard laboratory methods and molecularly characterized using staphylococcal chromosome cassette mec (SCCmec) typing and pulsed-field gel electrophoresis. Antimicrobial susceptibility testing was carried out using CLSI methods. The MRSA isolates studied displayed a total of 8 different pulsed-field gel electrophoresis patterns (types A to H) with predominance (73%) of pattern A (BEC-related). However, MRSA harboring SCCmec type IV were also identified, 3 (7%) of which were genetically related to the pediatric clone - USA800 (ST5-SCCmecIV). In addition, we found a considerable genetic diversity within BEC isolates. MRSA displaying SCCmecIV are frequently susceptible to the majority of non-β-lactam antibiotics. However, emergence of multiresistant variants of USA800 was detected. |
| Databáze: | Directory of Open Access Journals |
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