Bioassay for total serum bioactivity of Atractylodes lancea

Autor: Kesara Na-Bangchang, Anurak Cheoymang, Nadda Muhamad, Inthuon Kulma
Jazyk: angličtina
Rok vydání: 2023
Předmět:
Zdroj: Journal of Advanced Pharmaceutical Technology & Research, Vol 14, Iss 1, Pp 51-55 (2023)
Druh dokumentu: article
ISSN: 2231-4040
0976-2094
DOI: 10.4103/japtr.japtr_431_22
Popis: The study aimed to establish a bioassay for total bioactivity of Atractylodes lancea (AL) in human serum samples. Inhibition of bacterial growth (Staphylococcus aureus ATCC 25923) was assessed using 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay. The calibration curve (0, 0.39, 0.78, 1.56, 3.13, 2.56, and 50 ng/ml) was linear with correlation coefficients >0.990. The limit of quantification (LOQ) was 1.66 mg/ml using 20-ml serum sample. The developed bioassay method meets the standard of the bioanalytical method for determination of serum bioactivity of AL.
Databáze: Directory of Open Access Journals
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