| Autor: |
Caliga Răzvan, Lucian Maniu Călin, Mihăşan Marius |
| Jazyk: |
angličtina |
| Rok vydání: |
2016 |
| Předmět: |
|
| Zdroj: |
Open Life Sciences, Vol 11, Iss 1, Pp 71-77 (2016) |
| Druh dokumentu: |
article |
| ISSN: |
2391-5412 |
| DOI: |
10.1515/biol-2016-0009 |
| Popis: |
The activity of purified Fe3+ containing horseradish peroxidase (HRP) was recorded while the enzyme was exposed to two low frequency pulsed electromagnetic fields (ELF-EMFs) with different frequencies: 50 Hz/2.7 mT and 100 Hz/5.5 mT. No statistically significant difference in the Michaelis constant (Km) values between the control enzyme and the enzyme exposed to any of the two ELF-EMF used could be detected (53 ± 11 μM vs 67 ± 10 μM vs 42 ± 11 μM respectively), indicating that upon short exposure times, there are no large structural alterations of the enzyme that affect the substrate affinity. A 50 Hz/2.7 mT EMF did however, cause a significant decrease in the maximum rate (Vmax) value (from 3.31 ± 0.17 μmoles/min to 1.6 ± 0.19 μmoles/min, p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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