New insights into the regulation of TGF‐β/Smad and MPK signaling pathway gene expressions by nasal allergen and methacholine challenge test in asthma

Autor: Michał Gabriel Panek, Michał Seweryn Karbownik, Karol Maksymilian Górski, Marcelina Koćwin, Grzegorz Kardas, Mateusz Marynowski, Piotr Kuna
Jazyk: angličtina
Rok vydání: 2022
Předmět:
Zdroj: Clinical and Translational Allergy, Vol 12, Iss 7, Pp n/a-n/a (2022)
Druh dokumentu: article
ISSN: 2045-7022
DOI: 10.1002/clt2.12172
Popis: Abstract Background Asthma is a heterogeneous chronic inflammatory disease of the bronchi, the course of which is significantly influenced by extrinsic factors (specific and non‐specific). Methods The aim of this study was to evaluate the effect of these factors represented by nasal allergen challenge (specific factors) and methacholine challenge test (non‐specific) on changes in mRNA expression of genes encoding the TGF‐β (TGF‐β1 and TGF‐β3)‒Smad (mitogen‐activated protein kinase 1/3 [MPK1/3], Smad1/3/6/7) signaling pathway in asthmatic patients. Results Seventy‐five subjects were included in the study, of whom 27 were applied an intranasal allergen provocation and 48 a methacholine provocation. There were 9 men and 18 women in the intranasal provocation group, and 17 men and 31 women in the methacholine test group. We found that both examined the types of challenges contributed to changes in the relative expression of genes of the TGF‐β (TGF‐β1 and TGF‐β3)‒Smad (MPK1/3, Smad1/3/6/7) signaling pathway in asthmatic patients. A decrease was noted for MAPK1, MAPK3, Smad3, Smad6, and Smad7 genes and an increase of up to 2.5 times for TGF‐β1 gene. Conclusions Our experiment allows us to conclude that the change in the mRNA expression of the TGF‐β1–MPK1/3 and Smad3/6/7 genes occurs after an intranasal allergen and bronchial methacholine challenge.
Databáze: Directory of Open Access Journals
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