| Autor: |
Ishii Masakazu, Shimizu Shunichi, Hagiwara Tamio, Wajima Teruaki, Miyazaki Akira, Mori Yasuo, Kiuchi Yuji |
| Jazyk: |
angličtina |
| Rok vydání: |
2006 |
| Předmět: |
|
| Zdroj: |
Journal of Pharmacological Sciences, Vol 101, Iss 2, Pp 174-178 (2006) |
| Druh dokumentu: |
article |
| ISSN: |
1347-8613 |
| DOI: |
10.1254/jphs.SCJ06001X |
| Popis: |
Abstract.: Intracellular ADP-ribose is an activator of TRPM2, which is a Ca2+-permeable channel and mediates H2O2-induced cell death, in the TRPM2-expressing rat β-cell line RIN-5F. We examined the effect of extracellular-added ADP-ribose on intracellular Ca2+ concentration in RIN-5F cells. ADP-ribose induced Ca2+ release from the thapsigargin-sensitive Ca2+ store, but not Ca2+ entry across the plasma membrane. A phospholipase C (PLC) inhibitor and a non-specific IP3 receptor inhibitor blocked its Ca2+ release. H2O2-induced Ca2+ entry through TRPM2 was not affected by extracellular ADP-ribose. These findings suggest that extracellular-added ADP-ribose induces Ca2+ release through the PLC-IP3 pathway and does not act as a TRPM2 activator. Keywords:: ADP-ribose, hydrogen peroxide, TRPM2 |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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