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A 50 days feeding trail was conducted to evaluate the efficiency of spent oleaginous yeast Lipomyces starkeyi by-product from wheat straw as a probiotic supplement on juvenile red sea bream (Pagrus major). Elements that were investigated includes physiological condition, digestive enzyme, haemato-biochemistry, antioxidant, immune and stress. 300 juvenile P. major (9.13±0.05 g) were randomly distributed into 200 L polyethylene tank (20 fish per tank) in triplicate of 5 treatments; CD1 control (0 g/kg supplement), D2 (1%, 10 g/kg supplement), D3 (1.5%, 15 g/kg supplement), D4 (2%, 20 g/kg supplement), D5 (2.5%, 25 g/kg supplement). The fish were fed twice daily and sampling for weight and length were conducted at 35 and 50 days of the feeding trial. Whole body samples, digestive organs and blood collection was done at the end of the feeding trial. The results of the combine parameters assessed by multivariate analysis revealed that fish group fed on D5 has significantly better performance followed by D4, D2 and D3 compared to the fish group fed the control diet D1 (ANOSIM, P |
