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Jing Yang,1,2,* Donghui Jia,3,* Jialu Qiao,2,* Ximing Peng,1 Chuchao Zhou,1 Yanqing Yang1,* 1Department of Plastic Surgery, Tongren Hospital of Wuhan University (Wuhan Third Hospital), Wuhan, 430060, People’s Republic of China; 2Department of Medicine, Jianghan University, Wuhan, 430056, People’s Republic of China; 3Department of Traumatology, The Third Hospital of Jianghan University, The Huangpi People’s Hospital of Wuhan, Wuhan, Hubei, People’s Republic of China*These authors contributed equally to this workCorrespondence: Chuchao Zhou; Yanqing Yang, Email chuchaozhou@sina.com; 202131051063@stu.jhun.edu.cnIntroduction: The photo-activated thermo/gas antimicrobial nanocomposite hydrogel, Gel/PDA@BNN6, is composed of the nitric oxide (NO) carrier N, N’-di-sec-butyl-N, N’-dinitroso-p-phenylenediamine (BNN6), photothermal (PTT) material polydopamine nanoparticles (PDA NPs), and methacrylate gelatin (GelMA). This hydrogel can release NO gas in a stable and controlled manner, generating a localized photothermal effect when exposed to near-infrared laser light. This dual action promotes the healing of full-thickness skin wounds that are infected.Methods: Gel/PDA@BNN6 was developed, and both in vitro and in vivo experiments were carried out to evaluate its structure, physicochemical properties, antibacterial effects, effectiveness in promoting infected wound healing, and biocompatibility.Results: Gel/PDA@BNN6 was successfully synthesized, exhibiting a porous three-dimensional lattice structure and excellent mechanical properties. It demonstrated highly efficient photothermal conversion, controllable nitric oxide delivery, strong bactericidal effects, and minimal cytotoxicity in vitro. In vivo, Gel/PDA@BNN6, when used with NIR therapy, showed significant anti-inflammatory effects, promoted collagen deposition, and stimulated vascular neoangiogenesis, which accelerated wound closure. Additionally, it displayed superior biocompatibility.Discussion: Gel/PDA@BNN6 has shown an explicit curative effect for infected wound healing, suggesting it has a good chance of being an antimicrobial dressing in the future.Keywords: Nitric oxide, photothermal therapy, infected wound healing, hydrogel, BNN6 |
