| Autor: |
Sofie Olsson Hau, Sara Wahlin, Sophie Cervin, Vilgot Falk, Björn Nodin, Jacob Elebro, Jakob Eberhard, Bruce Moran, William M Gallagher, Emelie Karnevi, Karin Jirström |
| Jazyk: |
angličtina |
| Rok vydání: |
2022 |
| Předmět: |
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| Zdroj: |
The Journal of Pathology: Clinical Research, Vol 8, Iss 1, Pp 65-77 (2022) |
| Druh dokumentu: |
article |
| ISSN: |
2056-4538 |
| DOI: |
10.1002/cjp2.238 |
| Popis: |
Abstract The outlook for patients with pancreatic cancer remains dismal. Treatment options are limited and chemotherapy remains standard of care, leading to only modest survival benefits. Hence, there is a great need to further explore the mechanistic basis for the intrinsic therapeutic resistance of this disease, and to identify novel predictive biomarkers. RNA‐binding motif protein 3 (RBM3) has emerged as a promising biomarker of disease severity and chemotherapy response in several types of cancer, including pancreatic cancer. The aim of this study was to unearth RBM3‐regulated genes and proteins in pancreatic cancer cells in vitro, and to examine their expression and prognostic significance in human tumours. Next‐generation RNA sequencing was applied to compare transcriptomes of MIAPaCa‐2 cells with and without RBM3 knockdown. The prognostic value of differentially expressed genes (DEGs) was examined in The Cancer Genome Atlas (TCGA). Top deregulated genes were selected for further studies in vitro and for immunohistochemical analysis of corresponding protein expression in tumours from a clinically well‐annotated consecutive cohort of 46 patients with resected pancreatic cancer. In total, 19 DEGs (p |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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