| Autor: |
Thanya Pranomphon, Álvaro López-Valiñas, Carmen Almiñana, Coline Mahé, Viviane Lopes Brair, Rangsun Parnpai, Pascal Mermillod, Stefan Bauersachs, Marie Saint-Dizier |
| Jazyk: |
angličtina |
| Rok vydání: |
2024 |
| Předmět: |
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| Zdroj: |
Biological Research, Vol 57, Iss 1, Pp 1-19 (2024) |
| Druh dokumentu: |
article |
| ISSN: |
0717-6287 |
| DOI: |
10.1186/s40659-024-00555-5 |
| Popis: |
Abstract Background In vitro embryo production is increasingly used for genetic improvement in cattle but bypasses the oviduct environment and exposes the embryos to oxidative stress with deleterious effects on further development. Here we aimed to examine the effect of oviduct epithelial spheroids (OES) on embryo development and quality in terms of morphology and gene expression during two co-culture times (4 days: up to embryonic genome activation at 8–16 cell stage vs. 7 days: up to blastocyst stage) and under two oxygen levels (5% vs. 20%). Methods Bovine presumptive zygotes produced by in vitro fertilization (day 0) using in-vitro matured oocytes were cultured in droplets of synthetic oviductal fluid (SOF) medium with or without (controls) OES for 4 or 7 days under 5% or 20% oxygen (4 treated and 2 control groups). Cleavage rates were evaluated on day 2 and blastocyst rates on days 7–8. Expanded blastocysts on days 7–8 were evaluated for total cell numbers and gene expression analysis by RNA-sequencing. Results Under 20% oxygen, blastocyst rates and total cell numbers were significantly higher in the presence of OES for 4 and 7 days compared to controls (P |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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