Anti-fibrotic effect of rosmarinic acid on inhibition of pterygium epithelial cells
| Autor: | Ya-Yu Chen, Chia-Fang Tsai, Ming-Chu Tsai, Wen-Kang Chen, Yu-Wen Hsu, Fung-Jou Lu |
|---|---|
| Jazyk: | angličtina |
| Rok vydání: | 2018 |
| Předmět: | |
| Zdroj: | International Journal of Ophthalmology, Vol 11, Iss 2, Pp 189-195 (2018) |
| Druh dokumentu: | article |
| ISSN: | 2222-3959 2227-4898 |
| DOI: | 10.18240/ijo.2018.02.02 |
| Popis: | AIM: To investigate the anti-fibrosis effect of rosmarinic acid (RA) in pterygium epithelial cells (PECs) to determine if RA is a potent agent for treating pterygium. METHODS: The PECs (1×104 cells/mL) were treated with 100 μmol/L of RA for 1, 3 and 6h. After RA treatment, the cell viability was determined by staining with acridine orange/DAPI and analysis via a NucleoCounter NC-3000. The protein expression levels of type I collagen, transforming growth factor beta-1 (TGF-β1), TGF-β type II receptor (TGF-βRII), p-Smad1/5, p-Smad2, p-Smad3, and Smad4 of the cell lysates were measured by Western blot analysis. RESULTS: The cell viability of PECs was significantly decreased after RA treatment (P |
| Databáze: | Directory of Open Access Journals |
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