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Objective: To investigate the effect of ursolic acid extracted from Hippophae rhamnoides L. on the expression of key proteins of hepatic farnesoid X receptor (FXR) signaling pathway in rats with alcoholic liver injury. Methods: The 6-week-old SPF SD rats were divided randomly into 4 groups: The normal control group, the alcohol model group, the ursolic acid control group and the ursolic acid+alcohol group, 9 mice in each group. The rats were administered by intragastric administration for eight consecutive weeks. The pathological changes of hepatic tissues in rats was observed by hematoxylin eosin (H&E) staining. The activities of alanine aminotransferase (ALT), aspartate aminotransferase (AST) and the content of total bile acid (TBA) in serum, and the content of triglyceride (TG) and total cholesterol (TC) in liver of rats were tested. The content of the serum cytokine including tumor necrosis factor-α (TNF-α), interleukin 1β (IL-1β), and interleukin 10 (IL-10) was detected by enzyme linked immunosorbent assay (ELISA). The expression of FXR signal pathway related proteins in liver tissue of rats was detected by Western blotting. Results: Compared with the normal control group, there were fat vacuoles of different sizes and a large number of inflammatory cells in the liver of the alcohol model group. The activities of ALT, AST, the levels of TNF-ɑ, IL-1β, the content of TBA in serum and the content of TG, TC in liver were increased significantly (P |
