Hoxa9 and EGFP reporter expression in human Embryonic Stem Cells (hESC) as useful tools for studying human development
| Autor: | Joan Domingo-Reines, Adolfo López-Ornelas, Rosa Montes, Tamara Romero, Jose Luis Rodriguez-Llamas, Rolando Lara-Rodarte, Federico González-Pozas, Veronica Ayllon, Pablo Menendez, Iván Velasco, Veronica Ramos-Mejia |
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| Jazyk: | angličtina |
| Rok vydání: | 2017 |
| Předmět: | |
| Zdroj: | Stem Cell Research, Vol 25, Iss C, Pp 286-290 (2017) |
| Druh dokumentu: | article |
| ISSN: | 1873-5061 1876-7753 |
| DOI: | 10.1016/j.scr.2017.08.004 |
| Popis: | HoxA9 is an evolutionarily conserved homeobox gene implicated in embryo development. To study the roles of Hoxa9 during human development we generated a transgenic H9 (hESC) line that overexpresses HoxA9 and the Enhanced Green Fluorescent Protein (EGFP), and a control H9 with a stable expression of the EGFP. The resulting H9-HoxA9-EGFP and H9-EGFP cell lines allow an efficient visualization of hESCs by fluorescent microscopy, quantification by flow cytometry and cell differentiation tracking. Both transgenic cell lines maintained the pluripotent phenotype, the ability to differentiate into all three germ layers and a normal karyotype. |
| Databáze: | Directory of Open Access Journals |
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