Characterization of a novel fungal immunomodulatory protein, FIP-SJ75 shuffled from Ganoderma lucidum, Flammulina velutipes and Volvariella volvacea
| Autor: | Ke-Di Shao, Pei-Wen Mao, Qi-Zhang Li, Liu-Ding-Ji Li, Yu-liang Wang, Xuan-Wei Zhou |
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| Jazyk: | angličtina |
| Rok vydání: | 2019 |
| Předmět: | |
| Zdroj: | Food and Agricultural Immunology, Vol 30, Iss 1, Pp 1253-1270 (2019) |
| Druh dokumentu: | article |
| ISSN: | 0954-0105 1465-3443 09540105 |
| DOI: | 10.1080/09540105.2019.1686467 |
| Popis: | FIP-SJ75, a novel fungal immunomodulatory protein gene, was shuffled from the genes of three different mushroom species: Ganoderma lucidum, Flammulina velutipes, and Volvariella volvacea. Based on the expression of FIP-SJ75 gene in Escherichia coli, recombinant FIP-SJ75 (rFIP-SJ75) was routinely confirmed, consisting of 115 amino acids, and six peptides were identified by LC/Q-TOF MS with a coverage rate of 84.3%. Bioactivity assay in vitro indicated that rFIP-SJ75 promoted the proliferation of RAW264.7 cells at a range of 1–8 μg/mL, and significantly activated RAW264.7 cells in a dose-dependent manner. Real-time PCR revealed rFIP-SJ75 obviously promoted pro-inflammatory genes (TNF-α and IL-6), but reduced the expression of anti-inflammatory genes (IL-10 and TGF-β1) at the transcriptional level. These data implied that rFIP-SJ75 possessed immunomodulatory activity in macrophages by promoting macrophage M1 polarization and initiating pro-inflammatory responses, which could lay the foundation for rFIP-SJ75 to become a stable immunomodulator resource for further research and potential applications. |
| Databáze: | Directory of Open Access Journals |
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