| Autor: |
Lin YANG, Xianwei ZHU, Minoru SUGA, Hiroaki SHINOHARA |
| Jazyk: |
angličtina |
| Rok vydání: |
2021 |
| Předmět: |
|
| Zdroj: |
Electrochemistry, Vol 89, Iss 1, Pp 7-11 (2021) |
| Druh dokumentu: |
article |
| ISSN: |
2186-2451 |
| DOI: |
10.5796/electrochemistry.20-00113 |
| Popis: |
Demand for novel drug screening methods which are characterized by simple, rapid and low cost is increasing now. In this study, the simple and rapid evaluation of the anti-allergic effect of natural medicines on the degranulation of a model mast cell (RBL-2H3 cells) was successfully achieved by two-dimensional surface plasmon resonance (2D-SPR) measurement. The each cell response was observed by SPR imaging upon model antigen, Albumin from bovine serum, 2, 4-Dintrophenylated (DNP-BSA) stimulation after anti-DNP IgE sensitization on RBL-2H3 cells. Here glycyrrhizic acid (GA) and isoliquiritigenin (ISL) were examined as degranulation inhibitors. After the pretreatment with 30 µmol/L GA or 50 µmol/L ISL, the reflection intensity increase at cell regions upon DNP-BSA stimulation was completely suppressed. In addition, it was shown that the suppression of reflection intensity increase at cell regions in the 2D-SPR observation upon DNP-BSA stimulation was dependent on the GA and ISL concentration in pretreatment as well as β-hexosaminidase assay. These results demonstrated that the suppression effect of GA and ISL on the degranulation of RBL-2H3 cells could be evaluated by 2D-SPR observation at cell regions in quick and simple manner. Our study further suggested that 2D-SPR observation of mast cell response might be applicable to screen anti-allergic components of natural products and useful to discuss its inhibitory effect in the intracellular signaling pathway of mast cell upon antigen stimulation. |
| Databáze: |
Directory of Open Access Journals |
| Externí odkaz: |
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